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Laser Assisted Hatching (LAH)


The laser assisted hatching is an advanced and sophisticated additional lab technique designed to improve the efficiency of assisted reproductive technology procedures such as IVF/ICSI.,

Usually, women older than 37 years and above tend to produce eggs with a harder Zonapellucida than younger women. The same can be implied in the case of women treated with higher doses of Follicle Stimulating Hormone (FSH). After fertilization of the egg, during the cleavage stage of an embryo (embryo’s progression through 2-cell, 4-cell, 8-cell and 16-cell stages), Zonapellucida hardens. This development is normal to keep the cells in the egg together. Cryo preserved embryos also have hard Zonapellucida .

However, to implant into the endometrium lining of the uterus, the embryo must break out of zona pellucida through a process known as hatching.

The above procedure happens in about 2 to 3 days of embryo transfer, at blastocyst stage. It is possible for zona pellucida to harden at a faster pace when cultured in IVF laboratory. In order to provide conducive environment for the embryo to hatch from its protective outer shell – zona pellucida and to ensure implantation, laser-assisted hatching technique is used.

This cutting-edge Laser technology from Hamilton Thorne, USA, with the LYKOS or ZILOS-Tkuses focusses infrared laser beam to remove the Zonapellucida in very precise increments and facilitates hatching.

What is the success rate of IVF/ICSI with the help of laser assisted hatching?

Using Laser Assisted Hatching increases the chances of embryo hatching and implantation by many folds, making IVF/ICSI successful.

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